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Introduction

GigaPep MP-90 is originated from fish skin of marine fishes (cod, salmon, etc.), of which collagen is extracted by specialized hydrolysis technology. It is an emerging environmental-friendly active ingredient for skin care. Fish collagen does not have the risks of foot-and mouth disease or mad cow disease such as pigs, cattle and sheep, or the concern of zoonotic diseases. Moreover, it contains higher content of Glycine, proline, hydroxyproline and other characteristic amino acids, which are important components of extracellular matrix of human skin.

Effect

  • Form a fine film on the skin, achieving immediate skin tightening effect
  • Activate and promote the growth of the fibroblast cells, slow down cell senescence and effectively reduce fine lines
  • Promote the skin microcirculation around the eyes, preventing pouch and dark circles
  • Promote cell division and proliferation, differentiation, repairing and wound healing; inhibit skin inflammation
  • Inhibit excessive thickening of the epidermis and protect the skin against damage induced by ultraviolet B radiation

Functional testing

Excellent activation effect for skin cells

The artificial skin module was placed in the petri dish, which was applied with 3% GigaPep MP-90 formula product. The test was conducted after 24-h incubation. The attached figure demonstrated the changes in cell number of the skin module applied with GigaPep MP-90 formula product within 72 h.

In in vitro cell experiment, GigaPep MP-90 exhibited the most significant effect on the proliferation of fibroblast cells.

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(SkinEthic skin module)

  • GigaPep MP-90
  • Control

x : Incubation time ( H )
y : Cell abundance ( number of cell )

  • GigaPep MP-90
  • Commercial product A
  • Commercial product B
  • Commercial product C

x : Concentration of collagen ( μg/mL )
y : Growthrate ( % )

GigaPep MP-90 can induce the growth of fibroblast cells for a long period in the cell experiment

Effect of 9-day incubation of the cells as shown below

Control
GigaPep MP-90
Commercial product A
Commercial product B
Wound repairing promotion test Engineering for wound repairing
Excellent activation effect for skin cells

A wound 0f 0.9 mm was made on the monolayer cells; the blank group and the group added thecommercial product A/ commercial product B/ GigaPep MP-90 after 30-hours incubation were tested with the microscopic observation of the cell proliferation and migration to evaluate the wound repairing effect.

Sample classification :
blank group / commercial product A / commercial product B / GigaPep MP-90

Wound sample
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Demonstration of a 0.9-mm wound on the monolayer cells

Control
GigaPep MP-90
Commercial product A
Commercial product B
Capability of defending against the harm caused by UV radiation

One of the harm induced by UV light is hyperepidermal formation (thickening)
Experimental conditions : UVB irradiation dose: 0.3mW/cm2
Experimental time : repetitive irradiation for 6 weeks (2 hour each time)

It is revealed from the test that GigaPep MP-90 plays a role of inhibiting excessive thickening of the epidermis, reducing the harm induced by the UV light to the skin.

Human trial

Purpose

Test the effect of GigaPep MP-90 on the human skin

Experimental method

Women with the age of 20-59 (10 people) are applied with body lotion containing 5% GigaPep MP-90. Detect and record : test the state before use, 4 weeks later and 8 weeks later.

Measurement index
  • Portrait observation ⇒ Wrinkles around the eyes
  • Microscopic observation ⇒ The skin texture, depth and breadth of the wrinkles
  • Cuticle staining ⇒ Metabolism
  • Moisture meter ⇒ Water content of the skin
  • Analysis of the wrinkle state ⇒ Appearance
The picture analysis is originated from woman of 45 years old
Depth wrinkles
Average of depth
Development
Flower
Marketing
Largest width
  • One
  • Two
Numbers of wrinkles in fix area

Position : The wrinkle state around the eyes

Analysis of the wrinkle state
Depth wrinkles

x : week y : µm

Average of depth

x : week y : µm

Largest width

x : week y : µm

Numbers of wrinkles in fix area

x : week y : µm

8 weeks later, the wrinkle state of all subjects got improved, among which the largest depth was reduced by 24%, the average depth was reduced 17%, the largest breadth was reduced by 12% and quantity of wrinkles was reduced by 33%. Particularly, maximum depth and average depth are significantly decreased. ( ** : p < 0.01 )

The skin texture and appearance
The picture analysis is originated from woman of 45 year old

Position : Cheek ( 50× )

The white parts on the figures represent grease and aged horniness showing poor skin condition and texture. After 8-week use of body lotion containing 5% GigaPep MP-90, there is significant reduction of the bright white spots, representing improved keratin metabolism and skin condition.

The effect map of the keratinocyte metabolism
The picture analysis is originated from woman of 45 year old

Position : Cheek ( 50× )

Before use, keratinocytes had significant accumulation and lack of water with irregular appearance. However, the keratin became transparent representing suitable water content and arrangement 8 weeks later. It further revealed that GigaPep MP-90 could improve the water content of the skin and promote the protection and metabolism of the skin.

Improvement of the water content

x : week y : µm

Before use, keratinocytes had significant accumulation and lack of water with irregular appearance. However, the keratin became transparent representing suitable water content and arrangement 8 weeks later. It further revealed that GigaPep MP-90 could improve the water content of the skin and promote the protection and metabolism of the skin.

The effect map of the keratinocyte metabolism

Test instrument:JANUS-Ⅱ multimedia facial detection system
Subjects:Woman (55 years old)
Use of the essence containing 3% GigaPep MP-90 around the eyes twice a day for 4 weeks.

As shown from the figure: the wrinkles around the eyes became shallow from deep with significant lightening after four-week use of the essence, exhibiting significant anti-wrinkle effect.

The effect map of the keratinocyte metabolism

Subjects:woman (38 years old)
Apply the essence containing 3% GigaPep MP-90 around the eyes, then shot the local image with an iPhone7 camera

Subjects : woman (55 years old)
Apply the essence containing 3% GigaPep MP-90 around the eyes, directly shot the local images after immediate applying, 10 min and 30 min after applying with a VIVO X7 camera.

Physio-chemical index

AppearanceWhite to light yellow powder
Loss on drying (%)< 8.0%
Ash (%)< 2.0%
pH value (10%)5.5-7.5
heavy metal (Pb)≤ 10 ppm
Arsenic (As)content≤ 2 ppm
Total plate count≤ 100 CFU/ml
Mold and yeast≤10 CFU/ml

Application

GigaPep MP-90 is applied into immediate wrinkle-removing product, anti-wrinkle essence, facial mask, eye cream and other anti-aging skin care products.

Suggested usage

0.5%-5.0% (depend on the product requirement)

Storage

Sealed for storage under room temperature and dry condition, avoid direct sunlight.

Package

10 KG/bag

Manufacturer

Giga Fine Chemical (TAIWAN)